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Scientist I: CLD Small Scale Bioreactor SME

An exciting opportunity is available for a Cell Line Development Scientist to join a growing start-up. With state-of-the-art facilities and a robust pipeline of upcoming projects, this role offers the chance to contribute to innovative research and development.

Responsibilities:

The purpose of this role is to develop and execute small scale (Ambr15 & 24/96 deep well) fed-batch methods to screen and validate cell lines as part an innovative multidisciplinary team that will develop the next generation of mammalian expression systems & cells lines to enable highly productive pharmaceutical bioprocesses and, ultimately, more cost effective Immunotherapeutics.

Author SOPs, training documents & COSHH forms as key deliverables

Train team members to a high degree of competence to ensure data integrity

Document research findings clearly in a lab book

Communicate regularly in group meetings and prepare and deliver presentations to project team and internal stakeholders

Along with team members, provide routine laboratory maintenance, including participating in equipment upkeep, cleaning rotas and laboratory consumables stock management

Shared responsibility for out-of-hours cell culture maintenance.

Ensure that laboratory health, safety and quality procedures are followed

Support the preparation of laboratory risk assessments and maintenance of health and safety protocols

Maintain the confidentiality of the project and protocols

Keep abreast of current technologies and relevant scientific literature to help identify opportunity for improvements and reduction of costs
Minimum Required Qualifications

BSc/MSc in Biochemistry, Molecular Biology or related field

BSc/MSc with 5+ years of industry experience or PhD with 2+ years’ experience
Essential Experience:

Extensive experience of working with small scale bioreactor systems including Ambr15

Experience of developing small scall mammalian cell culture fed batch models systems using deep well microplates

Experience of screening clonal cell lines for optimal performance in batch, fed-batch and perfusion bioreactor systems

Experience of optimising fed-batch methods for antibody bioproduction

Experience of working with bioprocess relevant cell lines including CHO and HEK293

Experience of performing analytical methods to characterise cell lines for high antibody titre, qP and CQA such as HPLC/UPLC & LC-MS

Application of Design of Experiments (DOE) to rationalise experiment design

Skilled in experimental design and troubleshooting

Experience of writing SOPs, training documents and risk assessment

Experience of training other team members to a high level of competency

Familiarity of mammalian cell line development including generation of GM/GE cell lines, single cell cloning and CRISPR / ORF library screens

Evidence of executing a range of molecular and cell biology assays to a high standard including FACS, qRT-PCR, Western Blotting & ELISA

Ability to work independently and as part of team

Experience of planning experiments with a given timeframe

Good numeracy, computer and communication skills
Desirable Experience:

Experience with automation and high-throughput technologies applied to mammalian cell line generation eg. Beacon, 96 deep well plate model systems & Ambr15

Familiarity of working with large datasets including transcriptomic / proteomic datasets

Experience of mammalian cell culture and related techniques including passaging, banking, transfection, nucleofection & genotyping

Experience of working safely with 3rd generation lentiviral plasmid systems within a CAT2.0 environment
Skills and personality:

Innovative mindset

Advanced problem-solving skill

Enthusiasm and initiative

Driven to deliver

Reliable and responsible

Able to multitask

Good time management and organisation skills

Excellent communication skills both written and spoken

Happy in a growing and multi-disciplinary team